| Abstract [eng] |
Rapid advance in biology and medicine leads to an increasing demand of knowledge about chemical composition and structure of related molecular compounds, their function and response to changing environment. Vibrational spectroscopy has been increasingly used for this purpose; however, its application in routine practice is still episodic. Two main reasons for this are: (I) analysis of biological samples is challenging due to issues related to sample preparation and spectral analysis and (II) samples of various nature and in various environments require consideration of the most suitable approach for their analysis. Depending on the method chosen, specific spectral analysis accounting for various phenomena, such as scattering, reflections, absorption from surrounding media, etc., has to be performed in order to extract full information about the sample. Accordingly, the main goal of this thesis is complementary application of vibrational spectroscopy methods for analysis of biomedical samples - urinary stones, urinary deposits and biological fluids. The work is focused on adapting the methods and data analysis techniques to obtain reliable and repeatable results. |
