| Abstract [eng] |
Direct laser writing (DLW) in transparent cross-linkable materials based on the non-linear interaction of light and matter is one of the most widely used methods for the fabrication of three-dimensional polymeric micro-structures. It ensures high spatial resolution and offers wide spectrum of processable materials as well as possibility to choose the object’s geometry and scale. These and other properties resulted in DLW fabricated structures being used in areas such as photonics, micro-optics, medicine and others. The presented experimental work was carried out paying particular attention on the specifics of the fabrication using different pre-polymers. The dependence of the fabricated elements’ spatial resolution on the laser beam polarization was determined. Reversible deformations arising in the polymeric structures when they are placed in various liquids as well as their applications for the construction of environmental sensors were investigated. Also, the possibility to broaden the functionality of the fabricated structures by combining several pre-polymers during different fabrication stages was demonstrated. Possible applications of structures, fabricated by DLW, for tissue engineering were considered. It was shown that DLW is suitable for the rapid fabrication of millimeter-scale micro-structured scaffolds. In vitro and in vivo experiments of such constructs proved their suitability for use in tissue engineering. In addition to this, the structuring experiments performed using biocompatible elastomer PDMS allowed to improve its 3D structuring throughput. In conclusion, the presented experimental work furthers the development of the DLW methods towards their use for the practical applications. |
