| Abstract [eng] |
Rita Verbylaitė The use of plastid DNA sequences for Pomoideae Focke subfamily phylogeny, species identification and population diversity studies SUMMARY In this study was investigated the use of plastid DNA sequences for phylogeny, species identification and population diversity studies. Rosaceae family plants were used in this experiment. For population diversity studies it was used Rubus idaeus, and for phylogeny research – different Pomoideae subfamily species. For species identification it was taken unidentified species root samples. TrnL-trnF plastid region sequences were used for phylogeny and plant species identification, and for population diversity studies it was used trnS-trnG plastid region sequences. During this study the most suitable DNA extraction method for Pomoideae subfamily plants were identified. Also it was shown, that the most suitable method to analyse phylogenetic data, such as observed in this study is the maximum parsimony method. The monophyletic origin of Pomoideae subfamily including Vauquelinia and Kageneckia were confirmed. The close relationships between Crataegus and Mespilus were obtained. However, no intra-specific variation within the Pomoideae genera according to trnL-trnF plastid region was observed, and the hypothesis of Mespilus canescens origin still needs more data to be confirmed or rejected. In the second part of this study it was confirmed that sequencing is useful and one of the most promising method for species identification. The use of this method is constantly expanding because of the accumulating data in public sequence databases. Population diversity studies haven’t revealed any population differences among seven investigated Rubus idaeus plants, which were taken from different Lithuanian populations. |
