Title Reduced graphene oxide/organic dye composites for bioelectroconversion of saccharides: application for detection of saccharides and α-amylase assessments /
Authors Butkevičius, Marius ; Gaidukevič, Justina ; Gurevičienė, Vidutė ; Razumienė, Julija
DOI 10.3390/bios13121020
Full Text Download
Is Part of Biosensors: special issue: Nanocomposite-based biosensors: recent advances and perspectives.. Basel : MDPI. 2023, vol. 13, iss. 12, art. no. 1020, p. [1-15].. ISSN 2079-6374
Keywords [eng] biosensor ; reduced graphene oxide ; PQQ-dependent glucose dehydrogenase ; bioelectrocatalysis ; α-amylase ; saccharides
Abstract [eng] In this study, PQQ-dependent glucose dehydrogenase (PQQ-GDH) was immobilized onto reduced graphene oxide (rGO) modified with organic dyes from three different classes (acridine, arylmethane, and diazo); namely, neutral red (NR), malachite green (MG), and congo red (CR) formed three types of biosensors. All three rGO/organic dye composites were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy. The impact of three rGO/organic dye modifications employed in bioelectrocatalytic systems on changes in enzyme activity and substrate selectivity was investigated. The highest sensitivity of 39 µA/cm2 was obtained for 1 mM of glucose when a rGO_MG/PQQ-GDH biosensor was used. A significant improvement in the electrochemical response of biosensors was attributed to the higher amount of pyrrolic nitrogen groups on the surface of the rGO/organic dye composites. Modifications of rGO by NR and MG not only improved the surfaces for efficient direct electron transfer (DET) but also influenced the enzyme selectivity through proper binding and orientation of the enzyme. The accuracy of the biosensor’s action was confirmed by the spectrophotometric analysis. Perspectives for using the proposed bioelectrocatalytic systems operating on DET principles for total or single monosaccharide and/or disaccharide determination/bioconversion systems or for diagnoses have been presented through examples of bioconversion of D-glucose, D-xylose, and maltose.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2023
CC license CC license description