| Title |
Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling / |
| Authors |
Dirvelytė, Eimina ; Bujanauskienė, Daina ; Jankaitytė, Evelina ; Daugelavičienė, Neringa ; Kisieliūtė, Ugnė ; Nagula, Igor ; Budvytytė, Rima ; Neniškytė, Urtė |
| DOI |
10.1186/s11658-023-00472-7 |
| Full Text |
|
| Is Part of |
Cellular & molecular biology letters.. London : BMC. 2023, vol. 28, iss. 1, art. no. 59, p. [1-23].. ISSN 1425-8153. eISSN 1689-1392 |
| Abstract [eng] |
The dynamics of phosphatidylserine in the plasma membrane is a tightly regulated feature of eukaryotic cells. Phosphatidylserine (PS) is found preferentially in the inner leaflet of the plasma membrane. Disruption of this asymmetry leads to the exposure of phosphatidylserine on the cell surface and is associated with cell death, synaptic pruning, blood clotting and other cellular processes. Due to the role of phosphatidylserine in widespread cellular functions, an efficient phosphatidylserine probe is needed to study them. Currently, a few different phosphatidylserine labelling tools are available; however, these labels have unfavourable signal-to-noise ratios and are difficult to use in tissues due to limited permeability. Their application in living tissue requires injection procedures that damage the tissue and release damage-associated molecular patterns, which in turn stimulates phosphatidylserine exposure. |
| Published |
London : BMC |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
