Title Paramyxoviridae šeimos virusų nukleokapsidės baltymų sintezė mielėse Saccharomyces cerevisiae ir jų panaudojimas diagnostikai
Translation of Title Synthesis of paramyxoviridae nucleoproteins in yeast Saccharomyces cerevisiae and their application in viral diagnostics.
Authors Juozapaitis, Mindaugas
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Pages 187
Keywords [eng] Paramyxoviridae viruses ; nucleocapsid protein ; synthesis ; yeast Saccharomyces cerevisiae ; diagnostics.
Abstract [eng] The aims of this study was to investigate synthesis of SeV, hPIV1, hPIV3, hRSV, NiV, HeV and MenV nucleocapside (N) proteins in yeast Saccharomyces cerevisiae, to determine properties of recombinant N proteins and evaluate the feasibility to use them in diagnostics. In this study it was demonstrated, that yeast S.cerevisiae is an excellent host for a high-level production of proteins SeV, hPIV1, hPIV3, hRSV, NiV, HeV and MenV N proteins as virus nukleokapsid-like particles (vNLP). The yeast-expressed hPIV1, hPIV3, hRSV, NiV, HeV and MenV vNLPs represent useful tools for the development of new virus detection systems and demonstrate the effectiveness of yeast as a host for generation of recombinant proteins organized in complex structures like human virus NLPs.
Type Doctoral thesis
Language Lithuanian
Publication date 2011