Title Paramyxoviridae šeimos virusų nukleokapsidės baltymų sintezė mielėse Saccharomyces cerevisiae ir jų panaudojimas diagnostikai /
Translation of Title Synthesis of paramyxoviridae nucleoproteins in yeast Saccharomyces cerevisiae and their application in viral diagnostics.
Authors Juozapaitis, Mindaugas
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Pages 187
Keywords [eng] Paramyxoviridae viruses ; nucleocapsid protein ; synthesis ; yeast Saccharomyces cerevisiae ; diagnostics.
Abstract [eng] The aims of this study was to investigate synthesis of SeV, hPIV1, hPIV3, hRSV, NiV, HeV and MenV nucleocapside (N) proteins in yeast Saccharomyces cerevisiae, to determine properties of recombinant N proteins and evaluate the feasibility to use them in diagnostics. In this study it was demonstrated, that yeast S.cerevisiae is an excellent host for a high-level production of proteins SeV, hPIV1, hPIV3, hRSV, NiV, HeV and MenV N proteins as virus nukleokapsid-like particles (vNLP). The yeast-expressed hPIV1, hPIV3, hRSV, NiV, HeV and MenV vNLPs represent useful tools for the development of new virus detection systems and demonstrate the effectiveness of yeast as a host for generation of recombinant proteins organized in complex structures like human virus NLPs.
Type Doctoral thesis
Language Lithuanian
Publication date 2011