| Title |
In vitro L-Glutamate detection in different brain regions by GluOxRGO/Pt biosensor / |
| Authors |
Razumienė, Julija ; Leo, Damiana ; Gurevičienė, Vidutė ; Šakinytė-Urbikienė, Ieva |
| Full Text |
|
| Is Part of |
Proceedings: Eurosensors XXXIV, Lecce, Italy, 10-13 September 2023.. Basel : MDPI. 2023, p. [1-2].. ISSN 2504-3900 |
| Keywords [eng] |
L-glutamate ; amperometric biosensor ; brain regions ; WT and mutated rats |
| Abstract [eng] |
In this study we developed and validated a biosensor that is sensitive to quantify Lglutamate in different regions of rat’s brain. We have proposed the biosensor consisting of semipermeable membrane with L-Glutamate oxidase immobilized in to albumin/reduced graphene oxide (RGO) matrix and Pt electrode (GluOxRGO/Pt). The GluOxRGO/Pt biosensor was validated to quantify L-glutamate in brain tissues, namely the cerebellum, brainstem, hippocampus, striatum, mesencephalon and prefrontal cortex of the wild type (WT), dopamine transporter knock out (DAT KO) and DAT KO heterozygous (HET) rats. By this study we prove different L-glutamate distribution in separate regions of brains determined by type of rats. |
| Published |
Basel : MDPI |
| Type |
Conference paper |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
