| Title |
Electronically preresonant stimulated Raman scattering microscopy in the visible / |
| Authors |
Choorakuttil, A. J.X ; Pruccoli, A ; Winterhalder, M. J ; Zirak, P ; Gudavičius, D ; Martynaitis, Giedrius ; Petrulionis, Dalius ; Samsonas, Danielius ; Kontenis, Lukas ; Zumbusch, A |
| DOI |
10.1063/5.0171725 |
| Full Text |
|
| Is Part of |
Applied physics letters.. Melville : AIP Publishing. 2023, vol. 123, iss. 16, art. no. 163701, p. [1-7].. ISSN 0003-6951. eISSN 1077-3118 |
| Keywords [eng] |
microscopy ; Raman spectroscopy ; electrooptical devices ; Raman scattering |
| Abstract [eng] |
We report an experimental scheme for stimulated Raman scattering (SRS) microscopy with excitation in the visible spectral region. This allows electronically preresonant (epr) SRS microscopy of a broad range of chromophores with sensitivities as low as 1 μM. Our experiment is based on two synchronously near-infrared pumped optical parametric oscillators (OPO). One of the outputs is modulated at a fourth of the repetition rate with a novel broadband electro-optical modulator. Using a combination of spectral focusing and tuning of the OPO, we show the recording of epr-SRS spectra over the whole range of molecular vibrations at a speed up to 20 times faster than classical wavelength tuning. The imaging capabilities of this setup are demonstrated with material scientific and cellular samples. |
| Published |
Melville : AIP Publishing |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
