| Title |
Anthropogenic activities and microbial populations: war, peace or adaptation? / |
| Authors |
Armalytė, Julija ; Lastauskienė, Eglė |
| DOI |
10.3390/proceedings2023092075 |
| Full Text |
|
| Is Part of |
Proceedings: International conference EcoBalt 2023 "Chemicals & Environment", Tallinn, Estonia, 09–11 October 2023.. Basel : MDPI. 2023, vol. 92, iss. 1, art. no. 75, p. [1-2].. eISSN 2504-3900 |
| Keywords [eng] |
aquaculture ; metagenome ; resistome ; sediment metagenome ; antibiotic resistance ; resistome profiles |
| Abstract [eng] |
Abstract: Aquaculture is one of the fastestgrowing food sectors in Central, North, and Eastern Europe. Freshwater farming is changing the biodiversity of the fishing ponds to fulfill industrial needs, and these changes can impact the adjusting water bodies. Precautions should be taken to protect the ecosystems and keep them sustainable. The main objective of this research was to evaluate the influence of intensive fish farming on the condition of fishery ponds themselves and the surrounding water ecosystems as well as to evaluate the possibility of transferring the pollutants and antibiotic resistance genes to both environment and the human hosts. Sediment samples and fish gut microbiome samples were collected during September 2019 and the Summer of 2020 in 3 locations in Lithuania: Fishery ponds, Simnas Lake upstream from the fishery ponds, and Dusia Lake – downstream from the fishery ponds. The heavy metals and antibiotic residues were measured in the samples. Genomic DNA was isolated from the samples by using ZymoBIOMICSTM DNA Miniprep Kit according to the manufacturer’s recommendations. The composition of the bacterial community was determined by nextgeneration sequencing (NGS) by scanning the amplicons of the bacterial 16S rRNA gene. The V3–V4 16S rRNA regions were chosen for sequencing because they are capable to detect both bacterial and archaea taxons with high resolution [1], [2]. NGS was performed by Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) on Illumina paired-end platform to generate 250 base pairs (bp) length paired-end raw reads. All the tested sediment samples did not show significantly elevated heavy metal concentrations and no substantial veterinary antibiotic pollution. From the antibiotic resistance genes tested, the presence of aminoglycoside and b-lactam resistance determinants as well as the presence of integrons could be of concern for the possibility of transfer to humans. The microbiome betadiversity analysis results clearly indicated the differences between the microbiota composition of all pond sediments and entrance point, treated as a clean area. |
| Published |
Basel : MDPI |
| Type |
Conference paper |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
