Title |
Inflammation-suppressing cornea-in-a-syringe with anti-viral GF19 peptide promotes regeneration in HSV-1 infected rabbit corneas / |
Authors |
Šimoliūnas, Egidijus ; Ruedas-Torres, Inés ; Jiménez-Gómez, Yolanda ; Edin, Elle ; Aghajanzadeh-Kiyaseh, Mozhgan ; Zamani-Roudbaraki, Mostafa ; Ašoklis, Rimvydas Stanislovas ; Alksnė, Milda ; Thathapudi, Neethi C ; Poudel, Bijay K ; Rinkūnaitė, Ieva ; Ašoklis, Kasparas ; Iešmantaitė, Monika ; Ortega-Llamas, Laura ; Makselis, Almantas ; Munoz, Marcelo ; Baltriukienė, Daiva ; Bukelskienė, Virginija ; Gómez-Laguna, Jaime ; González-Andrades, Miguel ; Griffith, May |
DOI |
10.1038/s41536-024-00355-1 |
Full Text |
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Is Part of |
npj Regenerative medicine.. Berlin : Nature Research. 2024, vol. 9, iss. 1, art. no. 11, p. [1-15].. eISSN 2057-3995 |
Abstract [eng] |
Pathophysiologic inflammation, e.g., from HSV-1 viral infection, can cause tissue destruction resulting in ulceration, perforation, and ultimately blindness. We developed an injectable Cornea-in-a-Syringe (CIS) sealant-filler to treat damaged corneas. CIS comprises linear carboxylated polymers of inflammation-suppressing 2-methacryloyloxyethyl phosphorylcholine, regeneration-promoting collagen-like peptide, and adhesive collagen-citrate glue. We also incorporated GF19, a modified anti-viral host defense peptide that blocked HSV-1 activity in vitro when released from silica nanoparticles (SiNP-GF19). CIS alone suppressed inflammation when tested in a surgically perforated and HSV-1-infected rabbit corneal model, allowing tissue and nerve regeneration. However, at six months post-operation, only regenerated neocorneas previously treated with CIS with SiNP-GF19 had structural and functional features approaching those of normal healthy corneas and were HSV-1 virus-free. We showed that composite injectable biomaterials can be designed to allow regeneration by modulating inflammation and blocking viral activity in an infected tissue. Future iterations could be optimized for clinical application. |
Published |
Berlin : Nature Research |
Type |
Journal article |
Language |
English |
Publication date |
2024 |
CC license |
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