Title Wide-field Stokes polarimetric microscopy for second harmonic generation imaging /
Authors Uribe Castaño, Leonardo ; Mirsanaye, Kamdin ; Kontenis, Lukas ; Krouglov, Serguei ; Žurauskas, Edvardas ; Navab, Roya ; Yasufuku, Kazuhiro ; Tsao, Ming-Sound ; Akens, Margarete K ; Wilson, Brian C ; Barzda, Virginijus
DOI 10.1002/jbio.202200284
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Is Part of Journal of biophotonics.. Weinheim : Wiley-VCH GmbH. 2023, vol. 16, iss. 5, art. no. e202200284, p. [1-12].. ISSN 1864-063X. eISSN 1864-0648
Keywords [eng] lung tumor margin imaging ; nonlinear optical polarimetry ; second harmonic generation microscopy ; second-order susceptibility ; wide-field microscopy
Abstract [eng] We employ wide-field second harmonic generation (SHG) microscopy together with nonlinear Stokes polarimetry for quick ultrastructural investigation of large sample areas (700 μm × 700 μm) in thin histology sections. The Stokes vector components for SHG are obtained from the polarimetric measurements with incident and outgoing linear and circular polarization states. The Stokes components are used to construct the images of polarimetric parameters and deduce the maps of ultrastructural parameters of achiral and chiral nonlinear susceptibility tensor components ratios and cylindrical axis orientation in fibrillar materials. The large area imaging was employed for lung tumor margin investigations. The imaging shows reduced SHG intensity, increased achiral susceptibility ratio values, and preferential orientation of collagen strands along the boarder of tumor margin. The wide-field Stokes polarimetric SHG microscopy opens a possibility of quick large area imaging of ultrastructural parameters of tissue collagen, which can be used for nonlinear histopathology investigations.
Published Weinheim : Wiley-VCH GmbH
Type Journal article
Language English
Publication date 2023
CC license CC license description