| Abstract [eng] |
OLGA RAKSA DNA Polymorphism Study in Wild Populations of Herb – Paris (Paris quadrifolia L.) from Lithuanian Using RAPD Markers Summary Genetic variability of plant herb – Paris (Paris quadrifolia L.) was investigated using Random Amplified Polymorphic DNA (RAPD) technique. In the present study, a total of 8 polymorphic RAPD bands generated by five random primers were used to assess genetic diversity among 139 genotypes representing nine Paris quadrifolia populations. RAPD analyses have shown 21,05% of polymorphic loci. An analysis of molecular variance (AMOVA) indicated that genetic variation is greater within populations (70%) than among populations (30%). The relatively high gene flow (Nm = 0,96) between the analyzed populations and lowest genetic variation among populations (ΦPT = 0,30) revealed the importance of sexual propagation to the spread of this plant. For the total sample, Shannon’s Information Index was 0,51 and Nei’s gene diversity 0,35. To persuade, that the results we have gotten in this study didn’t associate with amount of used primers and polymorphic loci, we have compared our results with the analysis of polymorphism of herb Paris results, where 7 (14 polymorphic loci), 10 (19 polymorphic loci) primers were used. Amount of used primers and amount of polymorphic loci used in the present study, doesn’t influence polymorphism level of plant herb – Paris (Paris quadrifolia L.). |
