| Abstract [eng] |
The objective of this study was to investigate the functional, morphological, and gene expression changes induced by retinal ischemia reperfusion in Brown Norway rats. This investigation comprised three experiments. The first experiment aimed to establish the optimal pressure of sodium chloride for inducing the retinal ischemia reperfusion model. The second experiment aimed to explore functional and structural changes after interruption and restoration of blood flow. Lastly, the third experiment aimed to identify changes in gene expression induced by the retinal ischemia reperfusion model. Optical coherence tomography and pattern electroretinography were used to monitor morphological and physiological changes at different time points, while quantitative real – time polymerase chain reaction was used to study gene expression changes. The findings showed that a sodium chloride pressure at 200 mmHg was successfully to induce the model, resulting in increased intraocular pressure and decreased ganglion cell count. Additionally, after the temporary interruption and subsequent restoration of blood flow, the entire retina and ganglion cell layer became thinner, and the ganglion cells' response to stimulus decreased. A decrease in ganglion cell count and a reduction in the retina's weight were also observed. These morphological and physiological changes progressed over time. After performing quantitative real-time PCR, the results demonstrate that retinal ischemic reperfusion induced changes in the expression of GFAP, Ccl12, Casp8, and Bcl6 genes. |
