| Abstract [eng] |
MIPs with an sufficient affinity for melamine determination was prepared using conducting Poly pyrrole as the active motif for immobilization and signal enhancement. Small-sized functional thin films were formed by electropolymerization of working Poly pyrrole. The sensor fabrication molecular imprint is composed of weak bond interactions between the target and non-imprinted polymer and imprinted polymer. Using 1M Sulfuric acid solvent, the target was extracted from the MIPs, leaving pockets for rebinding the target. The selective and specific interaction of the target with MIPs makes the sensor for wide applications, including melamine determination. The sensor can be regenerated with a short wash cycle in PBS and re-used in the right circumstances. MIPs have better stability than biological receptors, including antibodies and proteins, enabling the construction of durable devices. Despite its simplicity, electrochemical sensor applications require a better understanding of nanostructured materials that could be used for better utilization and excellent sensitivity. This electrochemical sensor was used in a real-world analysis of melamine that revealed a need for further optimization and sample purification before using it for research. The sensor was designed specifically for the usage in an aquatic environment with good reproducibility and stability with a covariance coefficient of 3.75% but is also highly selective compared to melamine–like analysts like caffeine, Xanthine, and Theobromine. This developed electrochemical sensor based on MIPs for melamine determination showed excellent sensitivity within 0.1-1.0 μM. Least square line and I (MA) = 35.3 logC+56.53(-log C)(μM), Where C, Melamine concentration and r = 0.9957 is the correlation coefficient. The computed LOD and LOQ for the MIP Sensor are 90 and 280 nM, respectively. |
