| Abstract [eng] |
The aim of this work was to isolate and identify microorganisms, to estimate their ability to assimilate the substances with a lot of chains with any number of carbon (C) atoms. There were collected the samples, from the surfaces of cheese polyethylene packing in the storeroom – fridge. In accordance with their morphology there were isolated 12 bacterial strains. There was estimated the bacterial strains dependence on the temperature. I can propose, that the bacterial strains belongs to the facultative psychrophyles (psychrotrophs). There were analyzed the physiological properties of the bacterial strain: the composition of the cell wall, sugar fermentation, gelatin hydrolysis, starch hydrolysis, casein hydrolysis, tryptophan degradation, oxidase and catalase tests. According to the results of these test, there was made the clusters analysis and was determined the similarity of the bacterial strains. There was estimated the ability to assimilate hydrocarbons as the fragments of polyethylene, because the bacterial strains were isolated from the polyethylene surfaces. According to the results, there was determined that the bacterial strains assimilated the tetradecane, hexadecane and docosane. It means that after a long time these bacterial strains would start to assimilate the polyethylene as a sole carbon source. There was chose up the S7 bacterial strain for the identification. According to the results of the 16 S RNA gene analysis, there was determined that the analysed bacterial strain belongs to the genus of Staphylococcus. |
