| Abstract [eng] |
Identification of lactic acid bacteria in the migrant mallard ducks Anas platyrhynchos intestinal tract by partial 16S rRNA gene sequence analysis and using culture-based techniques Klaidas VARNA Institute of Ecology of Vilnius University, Laboratory of Hydrobionts Ecology and Physiology, Laboratory of Population Genetics, Akademijos-2, Vilnius-21, 08412, Lithuania. In this study the lactic acid bacteria diversity of the intestinal tract content of the vernal and autumnal migrant mallard ducks (Anas platyrhynchos) from Nemuno delta has been investigated by molecular methods: polymerase chain reaction amplification and sequencing of partial 16S rRNA genes and using culture-based techniques. The investigation of the lactic acid bacteria of the migrant mallard ducks has been performed the first time. Autumnal migrant mallard ducks in the small intestine walls (from 1.2×107 until 2.1×107 c.f.u./g) and in their content (from 3.4×107 until 1.1×108 c.f.u./g have the greatest number of the lactic acid bacteria then vernal migrants (respectively from 3.2×106 until 4.8×106 c.f.u./g and from 1.0×107 until 2.2×107 c.f.u./g). In the small intestine walls and in their content of the autumnal and vernal migrant mallard ducks, dominated cocci-shaped lactic acid bacteria (respectively 65% and 83.5%, 81.4% and 91.6%), whereas rod-shaped was under (respectively 35% and 16.5%, 18.6% and 8.4%). Supposedly, that these defferences determine some factors: a long migration, period of incubate, different feeds and environmental conditions. Little number of the lactic acid bacteria in the small intestines of the vernal migrant mallard ducks suggest birds state of health, determine failure of non-immunic resistance and outspread of pathogenic microorganisms. Predominant lactic acid bacteria detected in the small intestine and in their content of the vernal and autumnal migrant mallard ducks by filogenetic analysis were genera of Enterococcus sp. (S_ab score was 0.863 ± 0.000) and Lactobacillus sp. (S_ab score was 0.883 ± 0.003) Also has been identified genus of Propionibacterium sp. (S_ab score was 0.989 ± 0.011). The data obtained in the present work suggest that these results may be used in making more comprehensive analysis of the lactic acid bacteria of the intestinal tract of the migrant mallard ducks (Anas platyrhynchos). |
