| Abstract [eng] |
VILNIUS UNIVERSITY LIFE SCIENCES CENTER INSTITUTE OF BIOSCIENCES Greta Krikštaponytė Master thesis THE CORRELATION OF BLOOD THIAMINE CONCENTRATIONS WITH POLYNEUROPATHY SCALE DATA IN PATIENS WITH WERNICKE'S ENCEPAHLOPATHY SUMARRY Aim of the research: To investigate the relationship between thiamine blood levels and polyneuropathy scale data in with Wernicke encephalopathy (WE) patients. Work objectives: 1. Perform blood thiamine concentration determination by HPLC. 2. Assess the level of Wernicke encephalopathy using the polyneuropathy scale. 3. To evaluate the correlation between polyneuropathy scale data and blood thiamine levels. Methodology: Determination of blood thiamine concentration using high performance liquid chromatography (HPLC). Object of research: patients with suspected WE - acute neurological disorder caused by vitamin B1 deficiency. Results and conclusions Patients had below-normal vitamin B1 levels before treatment (80–120 μg / L): a mean concen.tration of 40 μg / L (s = 17,5 μg / L) and 1150,81 μg / L (s = 601,4 μg / L) was determined after one day of treatment, a week later – 954,17 μg / L (s = 575,3 μg / L). Patients’ polyneuropathy condition was assessed using the "Assessment of Critical Polyneuropathy and Neuropathy" questionnaire, according to which polyneuropathy scales were prepared: the average scores of tendon reflexes was 1,15 points out of 2, muscle strength was 3,4 points out of 5, sensitivity was 1,15 points out of 2. After comparing the data of the polyneuropathy scale (after one day of treatment) with the results of blood thiamine concentration (before treatment), a strong negative correlation was found, R = –0.92: after estimating the changes in muscle strength R = –0.91; sensitivity - R = –0.95; tendon reflexes R = –0.91. Preliminary evaluations of scale data with vitamin B1 blood levels showed that low blood thiamine levels contributed to high polyneuropathy scale scores in all patients studied.  . |
