| Abstract [eng] |
With the growth of the use of pharmaceutical substances, there is increasing evidence of the release of drugs into the environment: drug residues are found in surface and underground waters, soil, animal tissues. A large proportion of pharmaceutical substances enter the environment through wastewater from urban treatment plants and improperly disposed of obsolete or used drugs. The aim of this master 's thesis is to determine the effect of pharmacological substances on the function of menthol cells. Objectives: meta-analysis of data on the effects of ibuprofen, paracetamol and diclofenac on aquatic organisms; to investigate the effect of diclofenac, paracetamol and ibuprofen on the survival of Nitellopsis obtusa cells; to investigate the effect of diclofenac and paracetamol on the electrophysiological response; to compare the sensitivities of menthol algae and other aquatic organisms to ibuprofen, paracetamol, and diclofenac. The effect of pharmaceutical substances on N. obtusa cells was studied in several stages: collection of the test substance, mortality studies, evaluation of the duration of exposure. Based on the data from the mortality study and the meta-analysis material collected, the effects of the menthol algae cells and other aquatic organisms used in the study were compared. The results of the electrophysiological examination were also evaluated. Comparing the dynamics of LC50 effective concentration (LC50) values obtained in mortality studies of different durations, it was found that the cell death rate is not different for different pharmaceuticals with increasing duration of exposure to pharmaceuticals. The effective concentration of diclofenac solution was found to be 60.3 ± 15.5 mg / l (mean ± sp), which caused depolarization of the 50% resting potential of N. obtusa cells during 90 minutes of exposure. Paracetamol induced a significantly lower depolarization of resting potential ( RP) by 26–38% when exposed to even the highest concentrations of 1000–3000 mg / l. The residual depolarization of paracetamol lower than that of diclofenac also showed a lower toxicity of this analgesic to menthol cells. |
