| Abstract [eng] |
Human bone marrow stem cells have the ability to differentiate into a variety of cells. This feature of stem cells has potential in regenerative medicine. Ion channels are important in maintaining homeostasis and are differently expressed in a variety of cells. The aim of this study was to find if the expression of functional L-type Calcium channels changes during differentiation of stem cells. This included study the effects of Transforming Growth Factor-1β3, Intetrleukin-1β, and electrical stimulation on L-type Calcium channel expression, cell resting membrane potential and input resistance. The method of whole-cell patch –clamp recordings from stem cells was used. We found that inward L-type Calcium currents are not present in the control stem cells. Electrical stimulation of stem cells does not result in L-type Calcium currents or changes in cell membrane potential and input resistance. Cells affected by Transforming Growth Factor-1β3 showed increase in L-type Calcium channel expression without changes in membrane potential or input resistance. Intetrleukin-1β treatment also increased L-type Calcium channel expression and caused membrane potential depolarization without affecting the input resistance. |
