| Abstract [eng] |
The aim of this study was to investigate application of reduced graphene oxide (rGO) and polyaniline (PANI) nanocomposite in fabrication of amperometric glucose biosensor. In this study PANI nanowires were synthesized using an interphase polymerization method. The diameter of the prepared nanostructured PANI nanowires was 118 ± 19 nm. The sucessfull synthesis of rGO was confirmed using X-ray diffraction (XRD) method. The optimal ratio of PANI and rGO was found to be 1:10, which allows to achieve the best analytical parameters of the glucose biosensor. The KMapp. of a biosensor was 74.4 ± 9.0 mmol L-1. Due to the wide linear detection range from 0.5 to 50 mmol L-1, the developed biosensor can be used for glucose concentration detection in the samples with a high glucose concentration or in which the glucose concentration can vary greatly. The developed glucose biosensor is suitable for detection of glucose concentration in human serum. Glucose oxidase enzyme, which was isolated from Aspergillus niger and was used for biosensor fabricaon, is characterized by high affinity to glucose. The developed biosensor was highly selective for glucose, because in the case of addition of other sugars to the solution analytical signal was not monitored. Ascorbic acid and uric acid in diluted human serum have a slight effect on glucose determination. |
