| Abstract [eng] |
In this work the literature review and experiments of 3D laser lithography and multiphoton polymerization are described. When using photosensitive material and femtosecond laser pulses, photochemical reactions can be initiated in the volume of material. As a result, monomers in the focus area merge into polymers so that confined 3D object can be formed in a liquid photoresin. In this work femtosecond laser lithography experiments were performed with elastomeric resin polydimethyl-siloxane (UV-PDMS, manufacturer ShinEtsu). During the experiments the goal was to determine if this material can be processed with femtosecond laser of 515 nm wavelength. Also, to investigate the optimal parameters (focusing, exposition dosage) for the micro-fabrication of the material mentioned before. 3D scaffolds of UV-PDMS were fabricated and their biocompatibility as indicator of suitability for tissue engineering applications was tested. During the researches the optimal parameters of quality results we achieved with the high numerical aperture objective (1.4 NA compared to 0.45 NA and 0.8 NA), when choosing the exposition dosage in the range from 240 J/cm2 to 360 J/cm2. Also there was noticed, that the scanning in different directions (opposite and the same) results in different symmetry of the fabricated object. The biocompatibility test results of fabricated 3D scaffolds out of UV-PDMS material showed positive cell viability results and suitability for tissue engineering applications. The carried out researches provides the basis for further experiments of elastomeric 3D scaffolds of UV-PDMS, including its micro-architecture and grown cells in vivo investigation. |
