| Abstract [eng] |
Effect of Bismuth Gallate on Streptococcus Mutans Biofilm Formation Streptococcus mutans bacteria are considered to be the most important factor in the development of dental caries. Virulence of these bacteria is determined by their ability to form biofilm on solid surfaces and produce organic acids leading to tooth decay and cavitation. The discovery of new substances capable to suppress the formation of biofilm has a great importance for the prevention of dental caries. Aim of the research. To determine the effect of bismuth gallate on the formation of S. mutans biofilm in vitro. The following tasks were set for the research: to determine the effect of bismuth gallate on the biomass, thickness and roughness of S. mutans biofilm as well as to evaluate the effect of this substance on pH changes of the biofilm. Methods. The colorimetric assay was applied to the evaluation of the biomass formed by S. mutans and the optical profilometry assay – for the thickness and roughness. The pH of the growth medium of S. mutans biofilm was measured using a microelectrode. Results. Bismuth gallate exhibits the inhibitory effect on the formation of S. mutans biofilm. 10 mg/ml bismuth gallate concentration reduced the biomass of biofilm by 60.5% compared to the control (p < 0.05). Moreover, this substance also significantly reduced the thickness of S. mutans biofilm by 74% and the roughness parameter of the surface by 74.4%, compared to the control (p < 0.05). However, according to the pH measurements, bismuth gallate does not protect from the formation of acids in S. mutans biofilm. Conclusions. Bismuth gallate suppresses the formation of S. mutans biofilm on solid (polystyrene, glass) surfaces. Although this substance does not protect from the formation of acids in the biofilm, it can be used in the prevention of dental caries as an ingredient of the products designed to cover the tooth surfaces. |
