| Abstract [eng] |
The study was conducted in the year 2018–2019 at Biochemistry Laboratory, Center of Laboratory medicine, Vilnius University Hospital Santaros klinikos. Research objective: to implement new IFCC compatible alanine aminotransferase, aspartate aminotransferase, ferritin and creatinine methods for laboratory quality improvement and estimate their performance characteristics. Research methods. 116 ALT and 118 AST samples were tested without P5P and with P5P methods. 42 ferritin samples were tested by turbidimetric and microparticle chemiluminescent immunoassay methods. 121 creatinine samples were tested by modified Jaffe and enzymatic methods, the glomerular filtration rate was calculated. The method comparison and bias estimation using patient samples according to CLSI guidelines were performed. Results and conclusions. The bias of ALT results between methods was -27,5%, the data of the ALT method with P5P imprecision analysis correspond to the known characteristics of the biological variation model according to the Westgard database. The bias of AST results between methods was -24%, the data of the AST method with P5P imprecision analysis correspond to the known characteristics of the biological variation model according to the Westgard database. The bias of ferritin results between methods was 14,8%, the CMIA method imprecision analysis established that the characteristics of the method correspond to the characteristics declared by the manufacturer. The bias of creatinine results between methods was 8,1%, the enzymatic method imprecision analysis established that the characteristics of the method correspond to the characteristics declared by the manufacturer. The newly implemented ALT, AST, ferritin and creatinine methods are suitable for use in the routine laboratory practice. |
