| Abstract [eng] |
Impact of Pasteurization on the Proteins of Breast Milk Thesis was completed at VšĮ VULSKF Vaikų ligoninė from September of 2018 to March of 2019. Volume of the work : 50 pages with 58 sources of literature used and 24 pictures included. Aim of the work: to analyze microbiological content and concentrations of bioactive proteins– lactoferrin and lysozyme of human milk in women who had a preterm birth (<32 weeks, <1500g weight) and in women who had a term birth and to evaluate the impact of pasteurization on analyzed proteins. Objectives of the work: 1. Evaluate if bacterial growth in human milk depends on pregnancy duration. 2. To analyze differences between proteins of human milk depending on pregnancy duration. 3. To indicate the impact of pasteurization on lactoferrin and lysozyme concentrations in human milk. Methodology of the work: In this study 42 human milk samples were collected. 22 were from mothers who gave preterm birth and 20 who gave term birth. Milk culture analysis was done to evaluate bacterial growth and its correlation with pregnancy duration. After that concentration dependance on prematurity of birth and impact of pasteurization on lactoferrin and lysozyme was evaluated by using immunoenzymatic method with commercial Human Lactoferrin ELISA ir CircuLex Human Lysozyme ELISA sets. Results and conclusions: Accepted amount of milk cultures were colonized by coagulase- negative Staphylococcus spp. (10^3-10^4 CFU/ml) and Staphylococcus aureus (<10^5 CFU/ml) bacterias. Their growth does not correlate with pregnancy duration. Lactoferrin (p= 0,198) and lysozyme (p= 0,375) concentrations do not correlate with prematurity of birth. Pasteurization of human milk decreases both lactoferrin and lysozyme concentrations from 2,9 mg/ml to 0,01 mg/ml (99%, p< 0,0001) and from 12,4 µg/ml to 7,8 µg/ml (37%, p< 0,0001) respectively. |
