Title Oxidation of 1,5-benzodiazepine oximes catalysed by peroxidases /
Translation of Title Oксидација оксима 1,5-бензодиазепина катализована пероксидазама.
Authors Kosychova, Lidija ; Rekovič, Lina ; Bratkovskaja, Irina ; Radveikienė, Ingrida ; Vidžiūnaitė, Regina
DOI 10.2298/JSC230520068K
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Is Part of Journal of the Serbian Chemical Society.. Belgrade : Serbian Chemical Society. 2024, vol. 89, iss. 2, p. 151-164.. ISSN 0352-5139. eISSN 0352-5139
Keywords [eng] HRP ; rCiP ; 1,3,4,5-tetrahydro-2H-1,5-benzodiazepines
Abstract [eng] Oxidation of 1,3,4,5-tetrahydro-2H-1,5-benzodiazepine oximes catal­ysed by horseradish peroxidase (HRP) and recombinant Coprinus cinereus per­oxidase (rCiP) was studied spectrophotometrically. The reaction rate depend­ences on the substrate and hydrogen peroxide concentrations were investigated; the values of apparent KM and Vmax, catalytic, oxidation and reduction cons­tants (kcat, kox and kred, respectively) were calculated. The reactivity constants for the reactions catalysed by rCiP were higher than those for the HRP. Since oximes can have different structures depending on pH, the influence of pH on the rate of oxidation of compounds was studied. The dependences of the oxid­ation rate of the investigated oximes on the pH of the buffer solution were det­ermined, and the pKa values of the amino acids of peroxidases responsible for the rate of catalysis were obtained. The HRP activity dependence on pH has a classical bell-shaped character, while rCiP dependence has a complex character.
Published Belgrade : Serbian Chemical Society
Type Journal article
Language English
Publication date 2024
CC license CC license description