| Abstract [eng] |
Out of fruits and berries there was analyzed and prepared around 230 spontaneous fermentations. Out of them was extracted 17 strains, characterised with activated biocidic, evaluated it´s immune and fungicidal properties, comparing it with yeast S. cerevisiae sensitive and killer strains. The temperatures and pH for the best killer toxin secretion of killer yeast strains and bacterial isolates were defined. It shows that toxins of the isolates can be used against plants, animals and humans pathogenic microorganisms: Candida spp., Fusarium spp., Aspergillus spp., Penicillium spp., Verticillium spp., Venturia spp. 1M yeast strain was found, which had features, necessary for wine yeast and fermentated apple juice quality parameters and was used for natural apple wine semimanufactures production in cooperative company „Vaisių sultys“. During this research it was the first time when the K2 killer preprotoxin gene was cloned successfully to plant Nicotiana tabacum L. and analysed its expression. |
