| Abstract [eng] |
Upconverting nanoparticles are an excellent tool for biomedical applications that can be used in both cancer diagnosis and therapy. To increase the biocompatibility and accumulation of these nanoparticles in cancer cells, it is necessary to modify the surface of the nanoparticles with appropriate surface coatings. Additionally, surface coating is important for the formation of the necessary protein corona around the particle, which affects the endocytosis mechanisms and intercellular nanoparticles distribution in cancer cells. The aim of this work was to investigate the interaction of differently coated upconverting nanoparticles with cancer cells and to investigate the mechanisms of accumulation of nanoparticles in cells. Research has shown that rare earth metal differently coated upconverting nanoparticles are biocompatible, the protein corona improves the colloidal stability of nanoparticles, and surface coatings affect the dynamics of accumulation in cells. Cellular accumulation is also influenced by both the cell surface proteome and the composition of the protein corona formed around the nanoparticles. Moreover, it was observed that the amount of polyethylene glycol in the surface modifications of the nanoforms is an important factor regarding their accumulation in cancer cells. A higher amount of polyethylene glycol in the nanoparticle coating directly correlates with higher accumulation dynamics in the cells. |
