| Title |
Bimodal effects on lipid droplets induced in cancer and non-cancer cells by chemotherapy drugs as revealed with a green-emitting BODIPY fluorescent probe / |
| Authors |
Polita, Artūras ; Žvirblis, Rokas ; Dodonova-Vaitkūnienė, Jelena ; Shivabalan, Arun Prabha ; Maleckaitė, Karolina ; Valinčius, Gintaras |
| DOI |
10.1039/d3tb02979d |
| Full Text |
|
| Is Part of |
Journal of materials chemistry B.. Cambridge : Royal Society of Chemistry. 2024, vol. 12, iss. 12, p. 3022-3030.. ISSN 2050-750X. eISSN 2050-7518 |
| Abstract [eng] |
Lipid droplets (LDs) are cytoplasmic lipid-rich organelles with important roles in lipid storage and metabolism, cell signaling and membrane biosynthesis. Additionally, multiple diseases, such as obesity, fatty liver, cardiovascular diseases and cancer, are related to the metabolic disorders of LDs. In various cancer cells, LD accumulation is associated with resistance to cell death, reduced effectiveness of chemotherapeutic drugs, and increased proliferation and aggressiveness. In this work, we present a new viscosity-sensitive, green-emitting BODIPY probe capable of distinguishing between ordered and disordered lipid phases and selectively internalising into LDs of live cells. Through the use of fluorescence lifetime imaging microscopy (FLIM), we demonstrate that LDs in live cancer (A549) and non-cancer (HEK 293T) cells have vastly different microviscosities. Additionally, we quantify the microviscosity changes in LDs under the influence of DNA-damaging chemotherapy drugs doxorubicin and etoposide. Finally, we show that doxorubicin and etoposide have different effects on the microviscosities of LDs in chemotherapy-resistant A549 cancer cells. |
| Published |
Cambridge : Royal Society of Chemistry |
| Type |
Journal article |
| Language |
English |
| Publication date |
2024 |
| CC license |
|
