| Title |
Metagenomic analysis of the microbiome composition of aphids Adelges (Aphrastasia) pectinatae (Hemiptera: Adelgidae) / |
| Authors |
Tamošiūnaitė, Gustė ; Havelka, Jekaterina ; Kuisienė, Nomeda |
| DOI |
10.15388/Proceedings.2024.46 |
| eISBN |
9786090710517 |
| Full Text |
|
| Is Part of |
Open Readings 2024. The 67th international conference for students of physics and natural sciences, April 23-26, 2024 : book of abstracts.. Vilnius : Vilniaus universiteto leidykla, 2024. p. 310.. eISBN 9786090710517 |
| Keywords [eng] |
Adelges (Aphrastasia) pectinatus ; microbiome ; endosymbionts ; 16S rRNA gene |
| Abstract [eng] |
Aphids of the genus Adelges Vallot, 1836 are insects that feed by phloem sap sucking on host plant species of the conifer family Pinaceae, posing significant threats as pests. Bacteriocyte endosymbionts play a crucial role in the biology and ecology of these insects, residing within the host organism and participating in mutualistic relationships. Two classes of symbiotic bacteria are known in Adelges species: Betaproteobacteria and Gammaproteobacteria. In order to profile these symbiotic bacteria, total DNA was extracted from Adelges (Aphrastasia) pectinatae (Cholodkovsky, 1888) specimens, and subjected to next generation sequencing of 16S rRNA gene amplicon. Amplicon-based metagenomics targeted V3-V4 variable regions of bacterial 16S rRNA gene. It was determined that the representatives of the phylum Pseudomonadota were the most abundant (93.1% of all sequences) in the microbiome of A. (A.) pectinatae. 16S rRNA gene sequences of Gammaproteobacteria constituted 76.7% of all sequences, while only 0.3% of all sequences have been assigned to Betaproteobacteria. Unexpectedly, a significant portion of the analyzed sequences have been found to belong to the class Alphaproteobacteria in the phylum Pseudomonadota, namely to the orders Hyphomicrobiales (prev. Rhizobiales) (9.0% of all sequences) and Sphingomonadales (5.3% of all sequences). To the best of our knowledge, this is the first report about the quite abundant representatives of Alphaproteobacteria in the microbiome of aphids. Further analysis is needed to determine the role of these bacteria in the microbiome of. A. (A.) pectinatae. |
| Published |
Vilnius : Vilniaus universiteto leidykla, 2024 |
| Type |
Conference paper |
| Language |
English |
| Publication date |
2024 |
| CC license |
|
