Title Towards a self-powered amperometric glucose biosensor based on a single-enzyme biofuel cell
Authors Kaušaitė-Minkštimienė, Asta ; Kaminskas, Algimantas ; Gayda, Galina ; Ramanavičienė, Almira
DOI 10.3390/bios14030138
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Is Part of Biosensors.. Basel : MDPI. 2024, vol. 14, iss. 3, art. no. 138, p. [1-14].. ISSN 0265-928X. eISSN 2079-6374
Keywords [eng] glucose biosensor ; single-enzyme biofuel cell ; self-powered biosensor ; glucose oxidase ; Prussian blue ; gold nanoparticles ; poly(1,10-phenanthroline-5,6-dione)
Abstract [eng] This paper describes the study of an amperometric glucose biosensor based on an enzymatic biofuel cell consisting of a bioanode and a biocathode modified with the same enzyme—glucose oxidase (GOx). A graphite rod electrode (GRE) was electrochemically modified with a layer of Prussian blue (PB) nanoparticles embedded in a poly(pyrrole-2-carboxylic acid) (PPCA) shell, and an additional layer of PPCA and was used as the cathode. A GRE modified with a nanocomposite composed of poly(1,10-phenanthroline-5,6-dione) (PPD) and gold nanoparticles (AuNPs) entrapped in a PPCA shell was used as an anode. Both electrodes were modified with GOx by covalently bonding the enzyme to the carboxyl groups of PPCA. The developed biosensor exhibited a wide linear range of 0.15–124.00 mM with an R2 of 0.9998 and a sensitivity of 0.16 μA/mM. The limit of detection (LOD) and quantification (LOQ) were found to be 0.07 and 0.23 mM, respectively. The biosensor demonstrated exceptional selectivity to glucose and operational stability throughout 35 days, as well as good reproducibility, repeatability, and anti-interference ability towards common interfering substances. The studies on human serum demonstrate the ability of the newly designed biosensor to determine glucose in complex real samples at clinically relevant concentrations.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2024
CC license CC license description