| Abstract [eng] |
During this work large molecular weight plasmids from Arthrobacter sp. 68b, A. rhombi PRH1 and VP3 bacteria were investigated as well as small plasmids from both A. rhombi strains. It was determined that genes encoding degradation proteins of phthalic acid, 2 methylpyridine and pyridine are located on plasmid p2MP (113 kb) in Arthrobacter sp. 68b. The degradation of phthalate, pyridine and its derivative is inducible process. It was proved that cells pre-grown with phthalic acid are able to utilise quinolinic acid. Monooxygenase, that cleaves pyridine ring between C2, C3 atoms, is induced by pyridine and 2 methylpyridine. The degradation pathway of mentioned compounds was proposed. Succinate semialdehyde and succinic acid are formed during utilisation. Genes, encoding 2-hydroxypyridine degradation proteins, are located on large molecular weight plasmid. The phenotype of large A. rhombi VP3 plasmid was not determined. Small plasmids from both A. rhombi strains were sequenced, open reading frames were determined and identified. Using the minimal replicon of small A. rhombi PRH1 plasmid, hybrid vectors pRMU824, pRMU824Km and pRMU824Tc were constructed for functional gene screening in Arthrobacter spp. and Rhodococcus spp. bacteria. |
