| Abstract [eng] |
Trees of the poplar genus Populus tremula and Populus tremuloides are very common in Europe and North America. The genetic diversity and relation of two Populus tremuloides and Populus tremula hybrids (growing in Lithuania, Kaunas district) and 50 their offsprings were studied using random amplified polymorphic DNA method. The aim of this study was to estimate genetic relation and diversity of immature Populus trees in vitro and their parents using RAPD. The tasks of this study were: 1) evaluate genetic relation and diversity of selected hybrid aspens, 2) evaluate suitability and efficiency of used primers for these aspen hybrids. Primers used in this study Roth 170-02, Roth 170-03, and Roth 170-10 were informative. The results of electrophoresis using these primers were always discovered. Moreover there were identified a lot of fragments (40) using mentioned primers. Therefore these primers were effective in genetic relation and diversity studies of analysed P.tremula and P.tremuloides hybrid aspens. However primers Roth 170-01 and 170-04 in this case were little informative. The primer Roth 170-08 wasn’t informative, so it was unsuitable for RAPD analysis. According to RAPD results it was found that offsprings of aspens were more related to their mother individual (no.9) than to their father individual (no.8). The most closely RAPD based genetic distance found between individuals no.39 and no.40 (GDxy=0.05714), no.2 and no.20 (GDxy=0.10000), and no.7 and no.10 (GDxy=0.10256). Genetically distant individuals found no.21 and no.31 (GDxy=0.78049), no.31 and no.46 (GDxy=0.77273). DNA bands that were identified of all individuals using primer Roth 170-03 were the h fragment (1100 bp), y fragment (1290 bp), and k fragment (1500 bp). |
