| Title |
Chimerism analysis in isolated cell populations after allogeneic hematopoietic stem cell transplantation in children / |
| Translation of Title |
Chimerizmo analizė atskirose ląstelių populiacijose po alogeninės kraujodaros kamieninių ląstelių transplantacijos vaikams. |
| Authors |
Rascon, Jelena |
| Full Text |
|
| Pages |
32 |
| Keywords [eng] |
chimerism ; hematopoietic stem cell transplantation ; cell populations |
| Abstract [eng] |
The doctoral thesis aimed to evaluate in a comparative way the benefit chimerism assessment in isolated cell populations (ICP) versus conventional monitoring in whole blood cells (WBC). The study was initiated in Pediatric Bone Marrow Transplant Service of Charité Children’s Hospital (Humboldt University, Berlin). Children suffered from: acute lymphoblastic leukemia (ALL), Fanconi anemia (FA) and adrenoleukodystrophy (ALD) were included into the study. Thereafter the doctoral thesis was accomplished at the Vilnius University where evaluation of the own experience of chimerism analysis in pediatric patients was performed. Chimerism was prospectively monitored in WBC and three cell subsets: CD3, CD19 and CD34. Cell populations were extracted from peripheral blood using immunomagnetic beads. Following polymorphisms of short tandem repeats between donor and recipient were compared. The analysis reveled that following myeloablative conditioning in patients with ALL and ALD analysis of ICP revealed persistent autologous hemopoiesis despite stable donor chimerism in WBC. In contrast after reduced intensity conditioning 45.5% of FA recipients had no evidence of autologous signals either in WBC, or in ICP. FA were found to develop stable long-lasting mixed chimerism associated with better overall survival. development of MC was related to the infusion of bone marrow but not to peripheral blood stem cells. Chimerism in ICP did no affected transplant outcome in any disease group. |
| Type |
Summaries of doctoral thesis |
| Language |
English |
| Publication date |
2009 |
