| Abstract [eng] |
High–performance liquid chromatography (HPLC) and/or ultra-performance liquid chromatography (UPLC) techniques coupled with tandem mass spectrometry (MS/MS) were developed for the determination of common antibiotics (chloramphenicol, sulfonamides and nitroimidazoles together with their metabolites) in food products. HPLC/UPLC and MS/MS conditions were optimized for the separation and detection of the drugs by varying common experimental parameters. Effective and reliable sample extraction and clean-up procedures based on liquid-liquid extraction and/or solid-phase extraction techniques were developed for milk, honey and egg matrices. Proposed methods were validated according to EU criteria applying the alternative validation approach and the obtained results showed that methods are selective, accurate, reproducible and sensitive enough. Finally, the developed methods were successfully applied for the monitoring of Lithuanian food products. |
