| Abstract [eng] |
The aim of dissertation work to investigate the anti-inflammatory effect of murine bone marrow hematopoietic stem cells and their migration in the BALB/c mouse contact hypersensitivity model in vivo. It was found that isolated hematopoietic cell populations had a significant anti-inflammatory effect and inhibited the edema. The studies showed that the most efficient (up to 66%) inhibition of foot edema was obtained when using the HSC population. For the first time the HSC population migration kinetics in the BALB/c mouse contact hypersensitivity model in vivo was investigated. We determined that cells of this population can be found in mice paw edema and liver after just one hour. A little bit later they are detected in the spleen. We did the HSC population quantitative migration kinetic studies and found that in case of foot inflammation there is a secondary migration of the transplanted HSC migrating from the bone marrow to the spleen hematopoietic niche. We have shown that these cells selectively migrate into the inflammation areas of the foot edema. Transplanted cells quantity in the samples of foot edema, as compared with the untreated foot, was more than 1000 times higher. A transplanted hematopoietic stem cell migration research during inflammation, carried out in this work, contributes to clarification of stem cell migration patterns in case of pathological processes. This is particularly important in ensuring a safe and effective stem cell application in practice. |
