| Abstract [eng] |
The present study aims at distinguishing social risk factors of high grade cervical squamous intraepithelial neoplasia (HSIL) of women residing in particular areas employing the method of questionnaire surveys. The following risk factors were identified: not getting vaccinated against flu, living in villages and other rural areas, primary or special secondary education, negligent treatment of cervix. It was established that unequal distribution of health knowledge within population (according to occupation and age) does not encourage regular visits to gynaecologist. Moreover, although the mortality rate in Lithuania is the highest in Europe, the health promotion about preventive measures against cervical cancer is not sufficient. Thus immediate diagnostic methods have to be employed to examine the risk social groups during the first visit to the gynaecologist. The methods of fluorescence spectroscopy and microarray with p16INK4a marking in HSIL diagnostics were employed. Also, the diagnostic parameters of supernatant autofluorescence excitation and emission were established: Excitation of supernatant sample with 355 nm wave, emission 405–424 nm. The mobile micro laser STA-01 created in Lithuania and spectrometer AvaSpec-2048TEC-USB2 Avantes are supposed to be the most suitable optical method allowing to distinguish High grade cervical squamous intraepithelial lesions. Within normal limits and HSIL groups are separated p<0,001 (AUC 0,961), sensitivity is 0.91, specificity is 1,000). Taking into account precision, the microarray method of distinguishing protein in supernatant employed in the study does not differ greatly from p16INK4a evaluation using sample immunohistochemical method. The array method is necessary to be improved in order to implement it in women’s self screening process. |
