| Title |
Internal epitope tagging informed by relative lack of sequence conservation |
| Authors |
Burg, Leonard ; Zhang, Karen ; Bonawitz, Tristan ; Grajevskaja, Viktorija ; Bellipanni, Gianfranco ; Waring, Richard ; Balčiūnas, Darius |
| DOI |
10.1038/srep36986 |
| Full Text |
|
| Is Part of |
Scientific reports.. Berlin : Nature Portfolio. 2016, Vol. 6, art. no. 36986, Art. No. 36986.. ISSN 2045-2322 |
| Keywords [eng] |
zebrafish genome ; in-vivo ; efficient ; system ; specification ; regeneration ; transposon ; network ; heart ; tbx18 |
| Abstract [eng] |
Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function. |
| Published |
Berlin : Nature Portfolio |
| Type |
Journal article |
| Language |
English |
| Publication date |
2016 |
| CC license |
|
