| Abstract [eng] |
The present study demonstrates that adult muscle-derived stem cells with unlimited proliferative potential in vitro are able to differentiate into myogenic, adipogenic, osteogenic and neurogenic lineages. Differences in susceptibility to apoptotic stimuli depending on cell differentiation status and direction were shown in muscle-derived stem cells. The results prove that protein kinase Akt and ERK, p38, MAPK promote survival of differentiated cells during apoptotic treatments. The role of JNK kinase in apoptosis may depend on cell differentiation stage and type of apoptotic stimuli. We have proved that JNK has a potential to regulate pro- and antiapoptotic Bcl-2 family protein level in this cells. We have also shown the ability of JNK to regulate pro-survival Akt kinase phosphorylation level in differentiated cells. In conclusion, our results show that modulation of Akt and MAP kinases activity in accordance with muscle stem cell differentiation stage and apoptotic treatment can enhance the survival of cells during chemotherapeutic treatment and improve the effectiveness of stem cell therapy. |
