Title Functional organization of 3D plant thylakoid membranes as seen by high resolution microscopy /
Authors Streckaitė, Simona ; Ilioaia, Cristian ; Chaussavoine, Igor ; Chmeliov, Jevgenij ; Gelžinis, Andrius ; Frolov, Dmitrij ; Valkūnas, Leonas ; Rimsky, Sylvie ; Gall, Andrew ; Robert, Bruno
DOI 10.1016/j.bbabio.2024.149493
Full Text Download
Is Part of Biochimica et biophysica acta - bioenergetics.. Amsterdam : Elsevier. 2024, vol. 1865, iss. 4, art. no. 149493, p. 243-255.. ISSN 0005-2728. eISSN 1879-2650
Keywords [eng] high-resolution fluorescence microscopy ; thylakoid membrane ; intact chloroplasts ; 3D thylakoid network ; stromal lamellae ; chlorophyll fluorescence
Abstract [eng] In the field of photosynthesis, only a limited number of approaches of super-resolution fluorescence microscopy can be used, as the functional architecture of the thylakoid membrane in chloroplasts is probed through the natural fluorescence of chlorophyll molecules. In this work, we have used a custom-built fluorescence microscopy method called Single Pixel Reconstruction Imaging (SPiRI) that yields a 1.4 gain in lateral and axial resolution relative to confocal fluorescence microscopy, to obtain 2D images and 3D-reconstucted volumes of isolated chloroplasts, obtained from pea (Pisum sativum), spinach (Spinacia oleracea) and Arabidopsis thaliana. In agreement with previous studies, SPiRI images exhibit larger thylakoid grana diameters when extracted from plants under low-light regimes. The three-dimensional thylakoid architecture, revealing the complete network of the thylakoid membrane in intact, non-chemically-fixed chloroplasts can be visualized from the volume reconstructions obtained at high resolution. From such reconstructions, the stromal connections between each granum can be determined and the fluorescence intensity in the stromal lamellae compared to those of neighboring grana.
Published Amsterdam : Elsevier
Type Journal article
Language English
Publication date 2024
CC license CC license description