| Abstract [eng] |
Comparison Of Conventional and Molecular Methods In Pediatric Diarrhea Diagnostics. Scientific research for masters theisis “Comparison Of Conventional and Molecular Methods In Pediatric Diarrhea Diagnostics” was performed by Monika Miškinytė at Vilnius University Santariškių klinikos childrens hospital, with advisory supervision by scientific supervisor dr. (HP) Audronė Eidukaitė. The aim of this study: To compare conventional methods used in routine infectious diarrhea diagnostics with a new, molecucal multiplex pathogen detection panel. In order to carry out the aim of the study, the following tasks were set: 1. To identify infectious agents causing gastroenteritis that were previously unidentified by conventional methods using a molecular multiplex pathogen detection panel. 2. To evaluate the frequency and distribution of pathogens, detected by molecular multiplex pathogen detection panel. 3. To evaluate the advantages of Luminex xTAG GPP in routine laboratory diagnostics of infectious gastroenteritis. Objects and materials used: Faecal samples, that were identified as negative to rotavirus, norovirus and adenovirus infections via conventional methods (n=138) of children under 5 years old were tested using the Luminex xTAG GPP (Luminex Molecillar diagbostics Inc., Canada). Results: 1. 54,3% (n=76) of the tested samples were identified as positive to at least one of the Luminex xTAG GPP pathogens. 2. Norovirus GII was the leading pathogen identified (28%), followed by Salmonella spp., Campylobacter spp. and C. difficile (12%, 9% and 7% respectively). 3. The results of this study clearly how a low sensitivity of immunochromatography based quick Norovirus tests, when compared to the Luminex xTAG GPP. 4. More than 1/3 of the tested smaples (36,8%) were identified as positive to pathogens that were not tested via conventional laboratory methods due to it not being requested by a physician. 5. Luminex xTAG GPP is sensitive, fast and effective in determening wide range of pathogens causing infectious gastroenteritis, diagnosing co-infections and improving laboratory diagnostics of accute infectious gastroenteritis in children. |
