| Abstract [eng] |
SUMMARY Assessment of the Suitability of Self-collected Samples for the Detection of Human Papillomavirus (HPV) DNA and Correlation Between HPV Infection and Other Sexually Transmitted Infections Cervical carcinoma is the second most common neoplasia in women worldwide that is associated with Human papillomavirus (HPV) infection. In order to diagnose the disease in early stages, it is crusual to implement cervical cancer screening program and to cover the widest possible part of the population. The aim of the Thesis: Assessment of the suitability of self-collected specimens for the detection of the DNA of Human Papillomavirus (HPV) and correlation between HPV infection and other sexually transmitted diseases, submit recommendations regarding the specimen used in the cervical cancer screening program. Objectives: 1. To identify HPV virus and its' genotypes in the self-collected specimens and specimens collected by gynecologists wile using RT-PCR method. 2. To assess the suitability of self-collected specimens for the detection of the HPV DNA while comparing the HPV types detected in the self-collected specimens and specimens collected by gynecologists. 3. To assess the correlation between HPV infection and persistent bacteria in the vagina. Methods. 100 women participated in the study. During the study various HPV types were detected in the vaginal self-collected specimens and a cervical specimen collected by a gynecologist. In order to do that, at first DNA was being extracted from the specimens and after that the RT-PCR was performed. The results acquired were compared in between in order to assess the suitability of the method. Results. Results of 78 % of the self-collected specimens and specimens collected by gynecologist were identical. Coincidence of another 13 % of the results was partial. 9 % of detected HPV types did not coincide at all, but only 3 % of these cases were false negative (HPV was not detected in the self-collected sample, but was present in the doctor collected specimen). Incidence of HPV infection goes down as women get older, HPV infection is statistically more probable for women, whose sexual life started at early age (earlier than 18 years old) and those who had more sexual partners (3 and more). There was detected no correlation between HPV and smoking, ways of birth controle, bacterial vaginitis or candidiasis. However, Gardnerella vaginalis was detected more frequently in HPV-positive women. Conclusions. 1. The 100 specimens were analysed using RT-PCR method. HPV virus types were detected and classified as high or low oncogenic risk. 2. After the assessment of suitability of self-collected specimens for the detection of the HPV DNA while comparing the HPV types detected in the self-collected specimens and specimens collected by gynecologists it was determined that self-collected specimens can be used aiming to detect HPV. It is recommended to perform the cervical cancer screening program using self-collected specimens. 3. It was determined that Gardnerella vaginalis were detected more frequently in HPV-positive women. No correlation with other sexually transmitted agents was determined. |
