| Title |
Sausmedžio (Lonicera L.) genties atstovų genetinės įvairovės ir filogenetiniai tyrimai DNR žymenų metodais / |
| Translation of Title |
Study on genetic diversity and phylogenetic relationships in genus Lonicera L. using DNA marker methods. |
| Authors |
Naugžemys, Donatas |
| Full Text |
|
| Pages |
168 |
| Keywords [eng] |
Lonicera L. ; RAPD ; cpDNA ; phylogenetic ; genetic diversity |
| Abstract [eng] |
The aim of this study was to examine genetic diversity of the Lonicera L. collection of the Botanical Garden of Vilnius University, carry out intra-specific and inter-specific phylogenetic analysis through methods of molecular markers, and compare sequencing results with homologous cpDNA sequences registered in databases. Investigations on genetic diversity and intra-specific taxonomy of the blue-berried honeysuckle (L. caerulea L.) for the first time were conducted using DNA marker methods (RAPD and sequencing of cpDNA non-coding regions). L. caerulea representatives were found to have high level of DNA polymorphism. A species-specific RAPD marker was identified and sequenced for this polymorphic complex species. Genetic lines of the blue-berried honeysuckle derived in the Vilnius University distinguish by unique genetic properties and can be used in breeding to increase genetic diversity of new cultivars. The sequencing of six cpDNA non-coding regions of twelve taxa samples of genus Lonicera L. was done. Phylogenetic trees based on RAPD markers and cpDNA sequencing data were similar. Phylogenetic analysis showed that subspecies of the blue-berried honeysuckle described in scientific literature do not form one claster, but group with other taxa of disputable status and form with them a monophyletic group. |
| Type |
Doctoral thesis |
| Language |
Lithuanian |
| Publication date |
2011 |
