Title The current state of realistic heart models for disease modelling and cardiotoxicity /
Authors Kistamás, Kornél ; Lamberto, Federica ; Vaičiulevičiūtė, Raminta ; Leal, Filipa ; Muenthaisong, Suchitra ; Marte, Luis ; Subías-Beltrán, Paula ; Alaburda, Aidas ; Arvanitis, Dina N ; Zana, Melinda ; Costa, Pedro F ; Bernotienė, Eiva ; Bergaud, Christian ; Dinnyés, András
DOI 10.3390/ijms25179186
Full Text Download
Is Part of Molecular sciences: Special Issue: Research on Skeletal and Cardiac Muscle Regeneration Mechanisms.. Basel : MDPI. 2024, vol. 25, iss. 17, art. no. 9186, p. 1-34.. eISSN 1422-0067
Keywords [eng] hiPSC-CM ; cardiomyocyte ; toxicology ; drug testing ; cardiomyocyte maturation ; heart-on-a-chip ; cardiac model ; disease modelling
Abstract [eng] One of the many unresolved obstacles in the field of cardiovascular research is an uncompromising in vitro cardiac model. While primary cell sources from animal models offer both advantages and disadvantages, efforts over the past half-century have aimed to reduce their use. Additionally, obtaining a sufficient quantity of human primary cardiomyocytes faces ethical and legal challenges. As the practically unlimited source of human cardiomyocytes from induced pluripotent stem cells (hiPSC-CM) is now mostly resolved, there are great efforts to improve their quality and applicability by overcoming their intrinsic limitations. The greatest bottleneck in the field is the in vitro ageing of hiPSC-CMs to reach a maturity status that closely resembles that of the adult heart, thereby allowing for more appropriate drug developmental procedures as there is a clear correlation between ageing and developing cardiovascular diseases. Here, we review the current state-of-the-art techniques in the most realistic heart models used in disease modelling and toxicity evaluations from hiPSC-CM maturation through heart-on-a-chip platforms and in silico models to the in vitro models of certain cardiovascular diseases.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2024
CC license CC license description