| Title |
Investigation of structure and spectral properties of photoluminescent gold nanoclusters and their effect on cancer cells / |
| Translation of Title |
Fotoliuminescuojančių aukso nanoklasterių struktūros, spektrinių savybių bei poveikio vėžinėms ląstelėms tyrimai. |
| Authors |
Matulionytė, Marija |
| Full Text |
|
| Pages |
66 |
| Keywords [eng] |
gold nanoclusters ; photoluminescence ; photostability ; cellular uptake ; cytotoxicity |
| Abstract [eng] |
In the past decade ultrasmall photoluminescent gold and silver nanoclusters composed of several atoms with sizes up to a few nanometres attracted considerable interest as a new class of molecule-like photoluminescent probes for biomedical diagnostics and therapy. Optical properties and interaction with cells and organisms define the efficacy and applicability of nanoparticles in biomedical diagnostics and therapy. In this dissertation size, spectral properties, photostability under irradiation with UV/blue light, cellular uptake, cytotoxicity, and intracellular generation of reactive oxygen species of bovine serum albumin-encapsulated (BSA-Au NCs) and 2-(N-morpholino) ethanesulfonic acid (MES)-capped photoluminescent gold nanoclusters (Au-MES NCs) are presented. It was shown that photoluminescent BSA-Au and Au-MES NCs are not stable under irradiation with UV/blue light, changes in spectral properties showed degradation of Au NCs. In vitro experiments showed that photoluminescent BSA-Au NCs accumulated in vesicles at the perinuclear region in MCF-7 and MDA-MB-231 cells and did not induce significant cytotoxic effect. In contrast, photoluminescent Au-MES NCs distributed diffusely in cells including cell nuclei possibly due to ultrasmall size. This led to increased cytotoxicity and intracellular generation of reactive oxygen species in cancer cells. |
| Dissertation Institution |
Vilniaus universitetas. |
| Type |
Summaries of doctoral thesis |
| Language |
English |
| Publication date |
2017 |
