| Abstract [eng] |
In this study, TMPRSS2:ERG fusion transcripts were detectd in 58.86% of prostate cancer (PCa) tumour tissues, T1/E4 (46.62%) was the most predominant fusion type. Occurrence of the TMPRSS2:ERG rearrangement was accompanied by increased production of ERG, revealed increased production of the SPINK1 transcript in the subgroup of TMPRSS2:ERG fusion-negative cases. Our results demonstrated that the expression of TMPRSS2:ERG or TERT (catalytic subunit of telomerase) as a single biomarker was not highly informative for prediction of biochemical recurrence in PCa. However, the combination of both biomarkers showed significant association with the outcome of the disease. We successfully identified the TMPRSS2:ERG and TERT transcripts in catheterized urine of PCa patients. The detection rate of TMPRSS2:ERG reached 16.39% and was slightly lower than other investigators found. In this study we analyzed multifocal PCa samples. We identified different gene expression and TMPRSS2:ERG fusion transcripts isoforms were in multifocal PCa samples. This study identified reduced expression of tumour suppressor genes (GSTP1, RARB, RASSF1 and ZAC) in PCa non-cancerous prostate tissues and TMPRSS2:ERG fusion-positive cases, but we did not get significant association. Based on microarray data we selected five miRNAs (hsa-miR-33b, hsa-miR-370, hsa-miR-149, hsa-miR-886-3p and hsa-miR-206) that were down-regulated in TMPRSS2:ERG fusion-positive samples. QPCR data from PCa samples and cell lines transfection data shows, that hsa-miR-149 expression depends on the TMPRSS2:ERG transcript. Our results of XMRV virus analysis are well in line with the other XMRV RT-PCR or nested PCR based studies. XMRV virus arose in laboratory and is not related to prostate carcinogenesis. |
