| Abstract [eng] |
Electrochemical sensors play an important role in various industries and applications in different areas, including environmental monitoring, healthcare, safety, and industrial processes. They can be used to detect and quantify specific chemical compounds or elements. The market for electrochemical sensors is expanding rapidly due to technological advancements, increased environmental regulations, and growing healthcare needs. Polypyrrole is a polymer often used to design electrochemical sensors because it combines electrical conductivity, electrochemical activity, and adaptability. This dissertation aimed to evaluate polypyrrole modification possibilities to develop an electrochemical sensor. The polypyrrole layer was modified with phenothiazine derivatives or molecular imprints. The thesis is based on five publications that discuss the modifications of the polypyrrole layer using phenothiazine derivatives such as methylene blue, azure A, thionine and other additives. The results have demonstrated that among the three phenothiazine derivatives, methylene blue is the most promising in the development of an electrochemical sensor, and the influence of polysaccharides such as heparin helps to improve the adhesion and durability of the layer. In the context of the COVID-19 pandemic, experiments were conducted to detect the spike protein of SARS-CoV-2. Analysis of the coefficients of the integrated Cottrell equation has proven useful in evaluating the adsorption of analytes or products on an electrode. The results demonstrated that polypyrrole modified by molecular imprinting can be applied to detect SARS-CoV-2 spike protein and the detection of methylene blue. |
