Title Potato cyst nematodes Globodera rostochiensis and Globodera pallida, and their chemoecological interactions with the host plant /
Translation of Title Bulviniai cistiniai nematodai Globodera rostochiensis ir Globodera pallida, jų chemoekologinių sąveikų su augalu šeimininku tyrimas.
Authors Čepulytė-Rakauskienė, Rasa
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Pages 40
Keywords [eng] Globodera spp. ; identification of potato cyst nematodes ; chemoecological interactions
Abstract [eng] Potato cyst nematodes Globodera rostochiensis and Globodera pallida are one of the most important solanaceous plant pests. Identification of potato cyst nematodes species is exposed to morphological similarities and overlapping morphometric measurements between species. Only modern molecular techniques allow more accurate identification of potato cyst nematode species. Hence, it is important to apply these techniques in order to reliably identify these species in Lithuania. Potato roots release chemical compounds into the soil which attract nematodes. None of the potato released active compound (or compounds) is identified yet. For this reason, detailed examination of nematode behavior as well as analysis of bioactive chemical compounds released by potato roots is essential. Identification of these chemical compounds may lead to the ability of disturbing normal nematode behavior. Inhibition of nematode chemoreceptor activities may also disrupt normal nematode behavior. Accordingly, this knowledge could be applied for biological control of these quarantine pests. The aim of the research was to establish the composition and prevalence of potato cyst nematode species in Lithuania, as well as characteristics of potato cyst nematode chemoecological interactions with the host plant. Morphological and for the first time in Lithuania molecular (PCR) analysis of potato cyst nematodes collected from soil samples from all 10 counties of Lithuania showed that only one species of potato cyst nematodes – G. rostochiensis is detected in Lithuania (common in all counties), and G. pallida is not detected. For the first time it was revealed that potato released non specific metabolite linalool and specific metabolite α–solanine are attractive to potato cyst nematode second stage juveniles. For the first time it was evaluated that zinc sulphate inhibits nematode chemoatraction to α–solanine. For the first time, comparative analysis of gas chromatography of potato root released chemical compounds showed that at least three chemical compounds other than linalool and α–solanine are present in potato root emissions.
Type Summaries of doctoral thesis
Language English
Publication date 2012