| Abstract [eng] |
Biosensors have recently undergone significant developments in their application and specificity, making them a valuable tool in fields such as the analysis of biochemicals, environmental monitoring, drug development, and medical diagnosis. DNA biosensors have several advantages over traditional biosensors, including high specificity, efficiency, and the ability to detect a wider range of analytes. DNA- biosensors have customisable biosensing functions which makes it a better candidate for next generation biomedical detections. Due to their great catalytic efficiency, enzymes are frequently employed in biosensing. While DNA biosensors are generally less expensive to produce than traditional biosensors, the cost of enzymes used in the analytical processes can be high. This research focused on immobilising proteinase K enzyme on different electrode surfaces namely MIP (Molecularly imprinted polymers) with cavities and NIP (Non-imprinted polymers) and compared with clean Gold (Au) surface. To achieve this, Proteinase K enzyme was immobilised on the above mentioend surfaces, enabling the detection of signals EIS (Electrochemical impedance spectroscopy) and CV (Cyclic Voltammetry systems). The results showed that MIP-modified Au surfaces are better for applications requiring efficient and consistent Proteinase K immobilization, optimizing electrode performance for bioelectronic applications such as biosensing and bio-catalysis. Conclusively, successful immobilisation was performed and the results were assessed leading to this conclusion. Further studies must be done to explore the mechanistic interactions and stability of Proteinase K immobilized on these surfaces to enhance their application potential. |
