| Abstract [eng] |
The increasing resistance of microorganisms to existing treatments is encouraging researchers to find new alternative and natural ways to combat diseases caused by them. Due to their abundance in the Earth's crust and their unique physical, chemical, magnetic and luminescent properties, rare earth elements have contributed to many technological advances and have become increasingly attractive to the public over the last decades. In this work, the effect of La(NO3)3 × 6H2O on Staphylococcus aureus and Candida guilliermondii microorganisms was investigated. The study determined the MIC of La(NO3)3 × 6H2O against S. aureus and C. guilliermondii using a 96-well plate. In addition, the lanthanum compound was combined with an electric shock to the cells with a pulsed electric field generator. ROS generation by fluorescence microscopy was also performed and the temporal effects of La(NO3)3 × 6H2O were monitored by measuring the optical density of the cells. Based on the results obtained, it can be concluded that La(NO3)3 × 6H2O is antimicrobial, and that combining this compound with electric shock is even more effective. Bacteria were more resistant to this compound (MIC 4 mg/ml) than yeast (MIC 3 mg/ml). The strongest antimicrobial effect for both microorganisms was observed at 15 kV 100 µs × 1. The study also showed that La(NO3)3 × 6H2O generates reactive oxygen species in both S. aureus and C. guilliermondii. |
