| Abstract [eng] |
This study evaluated the cytotoxic, genotoxic, and antigenotoxic effects of Ganoderma lucidum (Reishi) and Hericium erinaceus (Lion’s Mane) mushroom extracts on human peripheral blood mononuclear cells (PBMCs) from five healthy donors. Cytotoxicity was assessed using a dual acridine orange/ethidium bromide staining technique, showing no significant cell viability reduction. The genotoxic and antigenotoxic effects were measured using the alkaline comet assay. Both mushroom extracts induced some DNA damage, though the changes were not statistically significant. Certain doses (10, 20, and 40 µL of Lion's Mane and 40, 50, and 80 µL of Reishi) resulted in less DNA damage and were selected for further antigenotoxic assessment. Antigenotixic effects of mushroom extracts against H2O2-induced DNA damage were evaluated in the pre- and post-treatment groups. In pre-treatment, cells treated with 80 µL of Reishi extract showed slight DNA damage reduction. For Lion’s Mane, 10 µL decreased damage, while 20 and 40 µL increased it. Post-treatment with Reishi extracts improved DNA repair, with 80 µL being the most effective. Lion's Mane extracts also reduced DNA damage at 10, 20, and 40 µL concentrations. Overall, both mushroom extracts showed potential antigenotoxic properties, varying with concentration and treatment duration. Further research is needed to confirm these findings and their implications for DNA repair. |
